Prerequisite ===================================== The Genehunter plus executable (namely "ghp" ) should be accessible from the STepc working directory. Command Line Syntax ===================================== stepc [-f parameter_file] if the "parameter_file option is not specified, STepc looks for the file "param.dat" in current working directory. Input Files ==================================== STepc requires 3 input files: a marker file, a pedigree data file and a parameter file. The marker file and pedigree data file are in LINKAGE format, for detailed information please refer to the web page http://linkage.rockefeller.edu/soft/linkage/, section DATAFILE and section PEDFILE. Parameter file specifies following parameters used by STepc: 1.DATA_FILE(mandatory): the path to find the pedigree data file, it could either be a absolute path or a relative path to current working directory. e.g. DATA_FILE /home/guest/ped_file DATA_FILE ped_file (relative path, refer to the file "ped_file" in current working path ) 2.MARKER_FILE(mandatory): the path to find the marker file. it could either be a absolute path or a relative path to current working directory. 3. START_MARKER_LIST ... END_MARKER_LIST (mandatory): a list of markers to be tested by STepc. e.g. START_MARKER_LIST SNP20 SNP66 SNP45 SNP44 SNP43 SNP79 SNP78 SNP77 END_MARKER_LIST Note, the name of markers should be consistent with their description in MARKER_FILE (e.g. "3 2 # SNP44" ).The marker is ignored from the if corresponding name not presented in MARKER_FILE. 4. GHP_PATH (optional): the path to find Genehunter plus executable "ghp". If not specified, STepc searches environment variable PATH for ghp executable (usually, in /usr/local/bin , /usr/bin/ etc. ), if the attempt fails, STepc quits and outputs error message "GHP_PATH not defined". 5. PDF_PLOT [on/off] (optional): the option to plot test results in pdf files. * Comments in parameter file: the comment contents in each line starts with symbol "#" or "//", STepc ignores followed content until end of the line.